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. 2021 Oct 7;22:67. doi: 10.1186/s12865-021-00459-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — The influence of TLR2 activation on cytolytic function of peripheral and tumor-infiltrating CD8⁺ T cells from NC and GC patients. CD8⁺ T cells were purified from HLA-A2 restricted individuals, including NC (peripheral bloods, n = 6) and GC patients (peripheral bloods and tumor specimens, n = 17), and were stimulated with or without Pam3Csk4 for 12 h in the presence of anti-CD3/CD28. Cells were washed twice, and 10⁵ of stimulated CD8⁺ T cells were cocultured in direct and indirect contact with 5 × 10⁵ of AGS cells for 48 h. The percentage of AGS cell death in a direct contact coculture system and b indirect contact coculture system was calculated by measuring lactate dehydrogenase release in the supernatants. The columns indicated means, and the bars indicated standard deviation. Significances were determined by One-Way ANOVA, LSD-t test or paired t test