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. 2021 Oct 7;20:391. doi: 10.1186/s12936-021-03907-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Plasmodium 18S rRNA biomarker correlation between DBS samples and liquid samples. Serial dilutions of P. falciparum cultures containing 4 × 10⁷, 5 × 10⁵, 5 × 10⁴, 5 × 10³ and 5 × 10² parasites/mL and 1, 5, 10, 10, and 10 replicates were used to prepare DBS and liquid samples and tested by the standard assay (A, B). The correlation between liquid and DBS samples were > 0.96 (A). Bland–Altman plots displayed biases (DBS-liquid samples) < 0.4 log₁₀ parasites/mL blood (B). Leftover clinical samples were collected from seven infected individuals throughout the course of their treatment to prepare DBS and liquid samples (C). Bland–Altman plots displayed biases close to 0.2 log₁₀ parasites/mL blood. Pf P. falciparum, Pan pan-Plasmodium