Table 4.
Measurements and percentages of detection rates for varied concentrations of P. falciparum Armored RNA® in liquid and DBS samples by the simplified assay
| Pf Armored RNA® Dilution | Liquid samples (log10 copies/mL blood) | DBS samples (log10 copies/mL blood) | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Parasites/mL blood | Log10 copies/mL blood | Pf qRT-PCR | Pan qRT-PCR | Pf qRT-PCR | Pan qRT-PCR | ||||
| Mean | SD | Mean | SD | Mean | SD | Mean | SD | ||
| 2.4 × 105 | 9.2 | 9.0 | 0.2 | 8.9 | 0.3 | 8.6 | 0.3 | 8.6 | 0.3 |
| 2.8 × 104 | 8.3 | 8.5 | 0.2 | 8.0 | 0.2 | 7.7 | 0.4 | 7.7 | 0.3 |
| 2,8 × 103 | 7.3 | 6.9 | 0.1 | 7.0 | 0.2 | 6.7 | 0.3 | 6.6 | 0.2 |
| 2.8 × 102 | 6.3 | 5.9 | 0.1 | 5.9 | 0.2 | 5.6 | 0.4 | 5.7 | 0.3 |
| 2.8 × 101 | 5.3 | 4.8 (3/4*) | n/a | 4.9 | 0.2 | 4.8 (6/8*) | n/a | 4.8 | 0.3 |
| 1.7 × 101 | 5.1 | 4.8 (3/4*) | n/a | 4.8 | 0.2 | 4.7 (6/10*) | n/a | 4.6 | 0.4 |
| 7 | 4.7 | 0.0 (0/4*) | n/a | 4.1 | 0.3 | 3.4 (5/11*) | n/a | 3.2 (9/11*) | n/a |
Pf P. falciparum, Pan pan-Plasmodium
n/a not applicable (SD not calculated for replicates of < 100% detection rate)
SD reflects variation in two batches of samples measured by multiple operators; n = 4–11 samples per concentration level
*Average calculated for quantifiable samples only (numbers in parentheses are detected results/number of replicates tested)