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. 2021 Oct 7;20:391. doi: 10.1186/s12936-021-03907-8

Table 6.

Identification of positive DBS samples by a two-stage hierarchy pooling scheme (pool size = 3)

Pool ID Result of each pool (log10 copies/mL blood) Sample ID Result of each member (log10 copies/mL blood) Previously reported result
Pf qRT-PCR Pan qRT-PCR Pf qRT-PCR Pan qRT-PCR
1–15, 17, 19, 20, 22, 23, 26 ND ND n/a n/a n/a ND
Pool 16 5.35 5.43 Z076 ND ND ND
Z077 ND ND ND
Z078 5.97 6.16 Pf
Pool 18 3.87 3.57 Z082 ND ND ND
Z083 ND ND ND
Z084 3.87 4.17 Pf
Pool 21 ND 5.65 Z093 ND ND ND
Z094 ND 6.27 Non-Pf Plasmodium sp.
Z095 ND ND ND
Pool 24 ND 5.43 Z102 ND 6.03 Non-Pf Plasmodium sp.
Z103 ND ND ND
Z104 ND ND ND
Pool 25 5.02 5.23 Z105 ND ND ND
Z106 ND ND ND
Z107 5.41 5.86 Pf

Pf P. falciparum, Pan pan-Plasmodium

ND not detected, n/a not applicable