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. 2021 Oct 8;12:104. doi: 10.1186/s40104-021-00627-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Effect of HS and supplemental 25-OH-D₃ in broilers at d 39 on Ca and P homeostasis. Plasma A 25-OH-D₃, and B 24(R), 25-dihydroxyvitamin D₃ (24(R), 25-(OH)₂-D₃)_, C ratio 24(R), 25-(OH)₂-D₃ to 25-OH-D₃, and D calcium and phosphorus. mRNA abundance of E RT-PCR analysis for mRNA expression of vitamin D receptor (VDR), sodium-dependent phosphorus transport protein II (NaPi-IIb) and calbindin-1 in duodenum, as well as VDR, NaPi-IIa and calbindin-1 in kidney. Treatments Ctrl, PF and HS without common superscript are significantly different at P < 0.05. * denotes significant difference between HS and HS + 25-OH-D₃ at P < 0.05