Fig. 3.

Effect of HS and supplemental 25-OH-D₃ in broilers at d 39 on A gut permeability and intestinal barrier, B hematoxylin and eosin (H&E) staining (× 100), C villus height, D crypt depth, and E their ratio, mRNA abundance of F zonula occludens-1 (ZO-1), G claudin-1 and H mucin-2. Treatments Ctrl, PF and HS without common superscript are significantly different at P < 0.05. * denotes significant difference between HS and HS + 25-OH-D₃ at P < 0.05