Table 2.
The information of antibodies used for western blot, immunohistochemistry, and flow cytometry
| Antibodies | Reactivity | Dilution | Catalogue | Application |
|---|---|---|---|---|
| GAPDH | Human | 1:1000 | Cell Signal Technology, #5174 | WB |
| PARP1 | Human | 1:1000 | Abcam, #ab191217 | WB; IHC |
| PD-L1 | Human | 1:200;2ug/ml | Abcam, #ab205921 | WB; IHC |
| STING | Human | 1:1000 | Cell Signal Technology, #13,647 | WB |
| p-STING_S366 | Human | 1:1000 | Cell Signal Technology, #50,907 | WB |
| TBK1 | Human | 1:1000 | Cell Signal Technology, #3504 | WB |
| p-TBK1_S172 | Human | 1:1000 | Cell Signal Technology, #5483 | WB |
| IRF3 | Human | 1:1000 | Cell Signal Technology, #11,904 | WB |
| p-IRF3_S396 | Human | 1:1000 | Cell Signal Technology, #29,047 | WB |
| CD8 | Human | 1:250 | Abcam, ab93278 | IHC |
| IFN-γ | Human | 1:50 | Novus, #JM10-10 | IHC |
| CD3 and isotype | Human | 5 μl of antibody per test | Elabscience, E-AB-F1001E | Flow cytometry |
| CD4 and isotype | Human | 5 μl of antibody per test | Elabscience, E-AB-F1109I | Flow cytometry |
| CD8 and isotype | Human | 5 μl of antibody per test | Elabscience, E-AB-F1110D | Flow cytometry |
| PD-L1 and isotype | Human | 5 μl of antibody per test | Elabscience, E-AB-F1133E | Flow cytometry |
| IFN-γ and isotype | Human | 5 μl of antibody per test | Thermo Fisher Scientific, 48–7319-41 | Flow cytometry |
| CD3 and isotype | Mouse | 5 μl of antibody per test | Elabscience, E-AB-F1013J | Flow cytometry |
| CD4 and isotype | Mouse | 5 μl of antibody per test | Elabscience, E-AB-F1097C | Flow cytometry |
| CD8 and isotype | Mouse | 5 μl of antibody per test | Elabscience, E-AB-F1104D | Flow cytometry |
| IFN-γ and isotype | Mouse | 5 μl of antibody per test | Elabscience, E-AB-F1101E | Flow cytometry |