TABLE 1.
Effect of adding of GSH‐OEt to the recovery medium of vitrified‐warmed oocytes on their in vitro development to blastocysts after ICSI
| Conc. of GSH‐OEt (mM) | No. of oocytes sperm‐injected | No. (%) of oocytes that survived after warming | No. (%) of 2‐cell embryos | No. (%) of blastocysts/2‐cell embryos |
|---|---|---|---|---|
| Control 1 | 68 | ‐ | 66 (97.1)a | 58 (87.9)a |
| 0 | 64 | 64 (100.0)a | 54 (84.4)b | 39 (72.2)b |
| 0.5 | 37 | 37 (100.0)a | 33 (89.2)b | 25 (75.8)b |
| 1.0 | 36 | 36 (100.0)a | 32 (88.9)b | 23 (71.9)b |
| 3.0 | 37 | 37 (100.0)a | 22 (59.5)c | 14 (63.6)b |
Vitrified‐warmed oocytes were cultured in recovery medium with or without GSH‐OEt for 1 h up to ICSI.
For this treatment, 0.5, 1.0, and 3.0 mM GSH‐OEt were added into global total medium.
a,bDifferent superscripts indicate a significant difference (p < 0.05), based on Fisher's exact probability test.
1
Fresh oocytes were cultured in global total medium without GSH‐OEt for 1 h up to ICSI, used as controls.