Figure 4.

c-Myc promoted proliferation and inhibited migration and differentiation of HaCaT cells. (a, b) Overexpression (a) and knockdown (b) of c-Myc were confirmed by Western blotting. (c) HaCaT cells overexpressing c-Myc showed higher proliferation. (d) HaCaT cells overexpressing c-Myc showed decreased expressions of TGM1 and LOR. (e) HaCaT cells overexpressing c-Myc showed slower migration. (f–h) Knockdown of c-Myc abolished the high proliferation rate (f), decreased expressions of TGM1 and LOR (g) and slow migration (h) caused by 30 mM glucose. ^*p < 0.05, ^**p < 0.01. c-Myc^OE c-Myc overexpression, LOR loricrin, OE-NC the negative control of c-Myc overexpression, shMyc c-Myc knockdown, shMyc-NC the negative control of c-Myc knockdown, TGM1 transglutaminase 1