PCR assay of genomic DNA from actinomycete species with primers NB10 and NB11. (A) Lanes: 1, 100-bp ladder; 2, N. brasiliensis NCTC 10300; lanes 3 to 8, N. brasiliensis clinical isolates. (B) Lanes: 1, markers; 2, S. somaliensis ATCC 33201; 3, S. somaliensis ATCC 19437; 4, R. rhodochrous ATCC 13808; 5, R. chubuensis ATCC 33609; 6, R. erythropolis ATCC 04277; 7, R. equi ATCC 06939; 8, G. rubropertinctus ATCC 14352; 9, G. terrae ATCC 25594; 10, G. sputi ATCC 33610; 11, G. bronchialis ATCC 25592; 12, A. pelletieri ATCC 33385; 13, N. otitidiscaviarum ATCC 14629.