Figure 1. Experimental design testing the effectiveness of size sorting Malaise trap samples and pooling the fractions in different proportions.

(A) Dried specimens are sieved into four different size classes, homogenized and lysed with a buffer amount corresponding to specimen weight in the respective fraction. (B) Lysate is incubated and pooled in different amounts. Grey numbers above the bars indicate the relative proportions (%). All six lysate pools were generated in duplicate, each individual size fraction (S, L, M, XL) was also extracted in duplicate, and nine negative controls were included.