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. 2021 Sep 14;14:712609. doi: 10.3389/fnmol.2021.712609

FIGURE 3.

FIGURE 3

Validation of SST interneuron subtype abundances subsequent to Grin1-ablation by RNA in situ hybridization. (A) Examination of cortical Nos1-expressing Chodl-SST.1 subtype abundances by (Ai) in situ hybridization using Sst and Nos1 RNAscope probes from P20-25 somatosensory cortex, counterstained with DAPI. (Aii) Boxplots indicate Sst(+), Nos1(+), Sst(+):Nos1(+) or Sst(+):Nos1(–) cell counts. (B) Examination of hippocampal Reln-expressing SST.2-4 subtype abundances by (Bi) in situ hybridization using Sst and Reln RNAscope probes from P20-25 hippocampus, counterstained with DAPI. (Bii) Boxplots indicate Sst(+), Reln(+), Sst(+):Reln(+) or Sst(–):Reln(+) cell counts. n = 4–6 brains from each genotype for immunostaining; n = 2 brains (4–6 sections/brain) from each genotype for RNAscope. Error bars reflect SEM; two-tailed unpaired t-test, for statistical analysis.