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. 2021 Sep 28;4(12):e202101182. doi: 10.26508/lsa.202101182

Figure 2. Constitutive activation of ATFS-1 results in up-regulation of genes from multiple stress response pathways.

Figure 2.

(A, B, C, D, E, F, G, H) Genes that are up-regulated by constitutive activation of ATFS-1 were compared with previously published lists of genes involved in different stress response pathways, including the mitochondrial unfolded protein response (A, mitoUPR), the ER unfolded protein response (B, ER-UPR), the cytoplasmic unfolded protein response (C, Cyto-UPR), the DAF-16–mediated stress response (D), the SKN-1–mediated oxidative stress response (E), the HIF-1–mediated hypoxia response (F), the p38-mediated innate immune response (G), and antioxidant genes (H). In every case, there was a significant degree of overlap ranging from 22 to 51%. Grey circles indicate genes that are up-regulated by activation of the stress response pathway indicated. Turquoise circles indicate genes that are up-regulated in the atfs-1(et15) constitutively active gain-of-function mutant. The numbers inside the circles show how many genes are up-regulated. The percentage overlap is the number of overlapping genes as a percentage of the number of genes up-regulated by the stress response pathway. P-values indicate the significance of the difference between the observed number of overlapping genes between the two gene sets, and the expected number of overlapping genes if the genes were picked at random. Panel (I) shows an inclusive UpSetR plot displaying the overlap between up-regulated genes associated with each stress response pathway. Vertical bars indicate the number of genes in common (overlap) between gene sets indicated by the dots below. Horizontal black bars indicate the number of genes within each gene set. mitoUPR, mitochondrial unfolded protein response; ER-UPR, endoplasmic reticulum unfolded protein response; Cyto-UPR, cytoplasmic unfolded protein response; DAF-16, DAF-16–mediated stress response pathway; SKN-1, SKN-1–mediated oxidative stress response pathway; HIF-1, HIF-1–mediated hypoxia response pathway; innate immunity, p38-mediated innate immunity pathway; antioxidant, antioxidant genes. Stress pathway gene lists and sources can be found in Table S3. Lists of genes common to multiple stress response pathways can be found in Table S4.