FIG. 6.

Immature granulocyte-macrophage colony formation and TNF-α-induced apoptosis in NOD spleen cells. Spleen cells (10⁵) derived from 6-week-old BALB/c (A, B, E, and F) and NOD (C, D, G, and H) mice were mixed with 1.3% methylcellulose gel dissolved in culture medium and layered onto a bed composed of 0.53% agarose and culture medium. Colonies were scored 3 weeks after cell plating. Spleen cells were cultured in 1.3% methylcellulose gels containing (+) (lower panels) or not containing (−) (upper panels) TNF-α (10 ng/ml). Each experiment was done in duplicate.