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. 2021 Oct 8;16(10):e0258156. doi: 10.1371/journal.pone.0258156

Fig 2. HeT-A ribonucleoproteins interact with Polo kinase in early Drosophila embryos.

Fig 2

(A) Coimmunoprecipitation experiments were performed on extracts from 0–2-h-old spnE_GLKD embryos using an anti-HA antibody. The samples were separated by SDS-PAGE and analyzed by western blotting using the antibodies indicated on the right. The starting lysate (input) and immunoprecipitated (IP) probes are indicated; the antibodies used for co-IP are indicated above the IP lanes. (B-E) The images demonstrate the localization of HeT-A Gag or HeT-A RNA (green), Polo (phosphorylated form, red), and CP190 (magenta) in the control (B), twin (C), Ars2 (D) and spnE (E) GLKD at metaphase in syncytial embryos. A cartoon of cleavage stage embryo is shown above the images. (F) The localization of HeT-A RNA (green), Polo (red), and CP190 (magenta) at anaphase in the Ars2_GLKD syncytial embryos. The blue color indicates DNA.