Fig. 7. Diminished T_FH cell phenotype by Tox2 deficiency is CD4⁺ T cell intrinsic.

(A) Experimental protocol of the generation of mix BM chimera and H3N2 infection. The BM from C57BL/6 Tox2^+/+ and Tox2^−/− mice with the indicated congenic marker was transferred to irradiated C57BL/6 Tox2^+/+ mice intravenously. After more than 8 weeks, mice were intranasally infected with H3N2. mLNs and spleen cells were collected at days 0, 7, 14, and 28 after infection to analyze cell populations by FACS. (B and C) Frequency of PD1^hiCXCR5^hi GC T_FH cells in mLN (B) and spleen (C) at indicated time points. Representative flow data (left) and the dataset of 6 to 12 mice are shown (right). (D) Frequency of CXCR5⁺Foxp3⁺ T_FR cells in spleen at indicated time points. Representative flow data (left) and the dataset of seven mice are shown (right).