Table 2.
Veiy short telomeres and telomere loss in senescent and cancer cell lines.
| Cell line used in telomere characterization | Characterized Chromosome arm | Average length | <500bpe | Telomere lossf |
|---|---|---|---|---|
| IMR90-senescence | Total (Bulk) | 4.0 ± 1.0 (851) | 49(5.8 %) | 0 |
| UMUC3 | Total (Bulk) | 3.1 ± 1.3 (871) | 57(6.5 %) | 0 |
| LNCaP | Total (Bulk) | 3.0 ± 0.7 (959) | 16(1.7 %) | 0 |
| U20S | Total (Bulk) | 3.1 ± 3.5 (906) f | 158(17 %) | 165 |
| SK-MEL-2 | Total (Bulk) | 3.4 ± 3.5 (862) f | 92(11 %) | 43 |
| Saos-2 | Total (Bulk) | 4.3 ± 4.2 (862) f | 53(7.8 %) | 49 |
| IMR90-senescence | 9p | 4.4 ± 2.2 (32) | 3(9.4 %) | 0 |
| IMR90-senescence | 8q | 2.6 ± 2.5 (31) | 8(25.8 %) | 0 |
| IMR90-senescence | 14q | 3.0 ± 1.9 (26) | 7(26.9 %) | 0 |
| UMUC3 | 9p | 1.7 ± 1.5 (30) | 12(40 %) | 0 |
| UMUC3 | 8q | 1.0 ± 0.5 (31) | 15(48.4 %) | 0 |
| UMUC3 | 14q | 1.9 ± 1.4 (10) | 1(10 %) | 0 |
| LNCaP | 9p | 3.4 ± 2.9 (32) | 0 | 0 |
| LNCaP | 8q | 2.2 ± 1.6 (31) | 5(16.1 %) | 0 |
| LNCaP | 14q | 3.9 ± 2.0 (14) | 0 | 0 |
| U20S | 3q | 0 | 0 | 23(13.9 %) |
| U20S | 8q | 1.2 ± 2.1 (42)g | 15(9.5 %) | 13(7.9 %) |
| U20S | 21q | 3.2 ± 4.4 (25)g | 9(5.7 %) | 18(10.9 %) |
| SK-MEL-2 | 8p | 3.1 ± 32.8 (32)g | 5(5.4 % %) | 5(11.6 %) |
| SK-MEL-2 | 14q | 1.7 ± 3.0 (23)g | 8(8.6 % %) | 4(9.3 %) |
| SK-MEL-2 | 20q | 1.4 ± 2.3 (29)g | 12(13.3 %) | 4(9.3 %) |
| Saos-2 | 1P | 2.8 ± 4.6 (37)g | 5(10.2 %) | 6(11.5 %) |
| Saos-2 | 19q | 4.8 ± 5.3 (31)g | 1(2.0 %) | 12(23.1 %) |
End telomere loss: Events where some molecules in a specific sample have demonstrated lack of telomere-like signal at the end of chromosome. Some of these events manifest as additional internal telomere like sequences with blight label while some did not have any ITS.
ITS loss: Events where ITS signal loss is observed.
Total Telomere loss: This is defined as the total number of molecules where either loss of end telomeres signal is observed 01 loss of ITS signal is observed.
Telomere loss not included. Percentage is calculated out of the total telomeres<500bp.
Total telomere loss (end telomere loss, ITS loss). Percentage is calculated out of the total telomere losses.
Average of both terminal telomere ends and recombinant ends.