Fig. 3.

Representative SOX-2 labeled immunofluorescence images of cells cultured (A) in the presence of complete medium, after priming (three days in culture), and (B) on TCPS, three days post-priming, in the presence of complete medium. (C) Representative immunofluorescence images of NPC-like, star-shaped, and elongated cells on G-25 and G-100 after three days in culture post-priming, and stained for GFAP (astrocyte), βIII-tubulin (neuronal), and SOX2 (stemness) markers. (D) Representative immunofluorescence images of star-shaped and elongated cells on G-25 and G-100 gels, nine days post-priming, in complete medium. Primary antibodies for SOX2, β-III tubulin, and GFAP were used. Staining for SOX2 was not evident. (E) Representative immunofluorescence images of cells after nine days of culture on laminin coated TCPS in the presence of complete medium or maintenance medium. Primary antibodies for SOX2, β-III tubulin, and GFAP were used with appropriate secondary antibodies. Cultures were counterstained with DAPI for cell nuclei identification in all the cases. The number of stained cells for each marker were counted manually from the images and normalized to the total cells counted in each case; n > 200 cells/case. * indicates p < 0.05 between respective cases. Scale bar: 50 μm.