(A) Plasma concentration of TCA intermediates in humans (n = 8).
(B) Citrate concentration in cell culture medias (n = 3) and human plasma (n = 8) same as in (A).
(C) SLC13A5 mRNA expression in human tissues, from (Uhlén et al., 2015).
(D) SLC13A5 mRNA expression in cancer cells grown in normoxia relative to Huh7s (n = 3).
(E) Slc13a5 mRNA expression in primary rat neuron and astrocyte cells in normoxia relative to neurons (n = 3).
(F) Net citrate uptake flux in cancer cell lines over 48 h (n = 3).
Cit, citrate; α-KG, α-ketoglutarate; Suc, succinate; Fum, fumarate; Mal, malate. All data are presented as means ± SD. All data are representative of three sample replicates.