KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
One Shot Stbl3 E. Coli	ThermoFisher Scientific	Cat#C7373
Library Efficiency DH5α Competent Cells	ThermoFisher Scientific	Cat#18263012
Biological samples
Healthy human plasma	Thalacker-Mercer Lab; Gheller et al., 2021	N/A
Chemicals, peptides, and recombinant proteins
[U-13C6] Glucose	Cambridge Isotope Labs	Cat#CLM-1396
[U-13C5] Glutamine	Cambridge Isotope Labs	Cat#CLM-1822
[2,4-13C2] Citrate	Cambridge Isotope Labs	Cat#CLM-148
[1,5-14C2] Citrate	Morovak Inc.	N/A
Zinc Chloride	Sigma-Aldrich	CAS# 7646-85-7
Sodium Citrate	Sigma-Aldrich	CAS# 6132-04-3
Methoxylamine Hydrochloride	MP Biomedicals	Cat#02155405
MTBSTFA + 1% TBDMSCl	Regis Technologies	Cat#1-270143-200
BsmBI	New England Biolabs	Cat#R0580L
Critical commercial assays
Lipofectamine 3000	ThermoFisher Scientific	Cat#L3000008
KAPA HiFi HotStart Ready Mix	Kapa Biosystems	Cat#KK2602
iTaq Universal SYBR Green	Biorad	Cat#1725121
iScript Reverse Transcription Supermix	Biorad	Cat#1708840
RNeasy Mini Kit	QIAGEN	Cat#74104
Zero Blunt TOPO PCR Cloning Kit	ThermoFisher Scientific	Cat#450245
prepGEM gold buffer	Zygem	N/A
Experimental models: Cell lines
HepG2	ATCC	Cat#HB-8065
Huh7	Provided by M. Hermann, MIT, Cambridge, MA, USA	N/A
A549	ATCC	Cat#CCL-185
143B	ATCC	Cat#CRL-8303
MCF7	ATCC	Cat#HTB-22
HEK293FT	ThermoFisher Scientific	Cat#R70007
Primary rat cortical neurons	This paper	N/A
Primary rat astrocytes	This paper	N/A
Oligonucleotides
See Table S1	N/A	N/A
Recombinant DNA
LentiCRISPR v2 plasmid	Addgene	Cat#52961
pCMVR8.2	Addgene	Cat#12263
pMD2.G	Addgene	Cat#12259
Software and algorithms
MATLAB 2014b and 2016b	MathWorks	https://www.mathworks.com/products/matlab.html
Escher-Trace	Kumar et al., 2020	https://escher-trace.github.io/
GraphPad Prism 7.00	GraphPad	https://www.graphpad.com/scientific-software/prism/
INCA 1.5	Young, 2014	https://mfa.vueinnovations.com/
CRISPOR	Concordet and Haeussler, 2018	http://crispor.tefor.net/
NCBI BLAST	Agarwala et al., 2018	https://blast.ncbi.nlm.nih.gov/Blast.cgi