| Strain, strain background (Mus musculus) |
H2B-mCherryfl/fl
|
Jackson Laboratories |
JAX:023139RRID:IMSR_JAX:023139
|
|
| Strain, strain background (Mus musculus) |
Pax7Cre=ERT2
|
PMID:26792330
|
MGI:4436914 |
|
| Strain, strain background (Mus musculus) |
Myo10tm1c
|
PMID:29229982
|
MGI:6115837 |
|
| Cell line (Mus musculus) |
C2C12 |
ATCC |
ATCC No. CRL-1772RRID:CVCL_0188
|
|
| Transfected construct (Homo sapiens) |
tagRFPt-HRAS-CAAX(RFP-CAAX) |
This paper |
Evrogen # FP141NCBI NP_005334
|
Membrane-targeted RFP |
| Transfected construct (Homo sapiens) |
EGFP-HRAS-CAAX(GFP-CAAX) |
This paper |
Clontech#632,470NCBI NP_005334
|
Membrane-targeted GFP |
| Transfected construct (Homo sapiens) |
RFP-Myo10 |
This paper |
Addgene No. 54,631NCBI No. NP_036466
|
Full-length Myo10 |
| Transfected construct (Homo sapiens) |
RFP-Myo10ΔCBD |
This paper |
Addgene No. 54,631NCBI No. NP_036466
|
Myo10(aa 1–938) |
| Transfected construct (Mus musculus) |
pMyo10-mApple |
This paper |
Addgene No. 54,631 |
Reporter for Myo10 promoter activation |
| Transfected construct (Mus musculus) |
Myomaker |
PMID:26858401
|
NCBI No. NP_079652
|
|
| Transfected construct (Mus musculus) |
Myomixer |
PMID:29581287
|
NCBI No. NP_001170939
|
|
| Transfected construct (Mus musculus) |
Myomaker-F203 |
PMID:26858401
|
NCBI No. NP_079652
|
Flag-tagged Myomaker |
| Transfected construct (Mus musculus) |
Myomixer-Flag |
PMID:28569745
|
NCBI No. NP_001170939
|
Flag-tagged Myomixer |
| Genetic reagent (Mus musculus) |
Control shRNA lentiviral particles |
Sigma-Aldrich |
No. SHCLNV shRNA ID- SHC002 |
|
| Genetic reagent (Mus musculus) |
Myo10 shRNA lentiviral particles |
Sigma-Aldrich |
No. SHCLNV shRNA IDs- TRCN0000110606 TRCN0000375033 |
|
| Antibody |
Anti-Myo10 (Rabbit polyclonal) |
Sigma-Aldrich |
HPA024223 |
IF: (0.3 µg/mL)IB: (0.24 µg/mL) |
| Antibody |
Anti-Myo10 (Mouse monoclonal) |
Santa Cruz |
sc166720 |
IF: (2 µg/mL) |
| Antibody |
Anti-Laminin(Rat monoclonal) |
Acris Antibodies |
BM6064P |
IF: (1.25 µg/mL) |
| Antibody |
Anti-Myosin Heavy Chain(Mouse monoclonal) |
R&D Systems |
MAB4470 |
IF: (0.25 µg/mL)IB: (0.125 µg/mL) |
| Antibody |
Anti-mCherry(Chicken polyclonal) |
Novus |
NBP2-25158 |
IF: (1:2000) |
| Antibody |
Anti-MyoD(Mouse monoclonal) |
Thermofisher |
MA1-41017 |
IF: (5 µg/mL) |
| Antibody |
Anti-Myogenin(Mouse monoclonal) |
Novus |
NB100-56510 |
IF: (2 µg/mL) |
| Antibody |
Anti-TMEM8C/Myomaker(Rabbit polyclonal) |
Thermofisher |
PA5-63180 |
IF: (1 µg/mL) |
| Antibody |
Anti-Myomixer(Sheep polyclonal) |
R&D Systems |
AF4580 |
IF: (0.67 µg/mL) |
| Antibody |
Anti-Flag(Mouse monoclonal) |
Sigma-Aldrich |
F3165 |
IF: (1.6 µg/mL) |
| Antibody |
Anti-RFP(Rabbit polyclonal) |
Abcam |
ab62341 |
IB: (0.5 µg/mL) |
| Antibody |
Anti-GFP(Chicken polyclonal) |
Abcam |
ab13970 |
IB: (2.5 µg/mL) |
| Sequence-based reagent (Mus musculus) |
Myo10 Forward Primer |
This paper |
NCBI No.NM_019472
|
TTC CAC CGC ACA TCT TCG CCA TTG |
| Sequence-based reagent (Mus musculus) |
Myo10 Reverse Primer |
This paper |
NCBI No.NM_019472
|
CCC CGG GAT TCT GCC TCA CTA CTC |
| Sequence-based reagent (Mus musculus) |
Myh2 Forward Primer |
This paper |
NCBI No.NM_001039545
|
AGA ACA TGG AGC AGA CCG TG |
| Sequence-based reagent (Mus musculus) |
Myh2 Reverse Primer |
This paper |
NCBI No.NM_001039545
|
TCA TTC CAC AGC ATC GGG AC |
| Sequence-based reagent (Mus musculus) |
Gapdh Forward Primer |
This paper |
NCBI No.BC023196
|
AGC AGG CAT CTG AGG GCC CA |
| Sequence-based reagent (Mus musculus) |
Gapdh Reverse Primer |
This paper |
NCBI No.BC023196
|
TGT TGG GGG CCG AGT TGG GA |
