Table 2. Predicted donor-acceptor distances from MBP X-ray crystal structures.
| Donor | Acceptor | R0 (Å) | Apo vs. Holo | rpredicted,β-carbon (Å) | rpredicted,centroid (Å) |
|---|---|---|---|---|---|
| MBP-295Acd-Y307F-C | Cu2+-TETAC | 14.9 | Apo | 21.3 | 21.7 |
| MBP-295Acd-Y307F-C | Cu2+-TETAC | 14.9 | Holo | 12.8 | 12.1 |
| MBP-295Acd-Y307F-HH | Cu2+ | 12.2 | Apo | 18.7 | 17.1 |
| MBP-295Acd-Y307F-HH | Cu2+ | 12.2 | Holo | 10.9 | 7.8 |
| MBP-322Acd-C | Cu2+-TETAC | 14.9 | Apo | 13.4 | 11.2 |
| MBP-322Acd-C | Cu2+-TETAC | 14.9 | Holo | 17.7 | 18.8 |
rpredicted, β-carbon, and rpredicted centroid were determined from PDB structures 1OMP and 1ANF. The calculated R0 values and predicted distances for MBP-295Acd constructs are the same as for MBP-295Acd-Y307F constructs.