Extended Data Fig. 6. The structures of OAD MTBDs and their interactions with microtubule doublet.

(a) Synchronized positions of the OAD MTBDs bound to four microtubule protofilaments in MTBS1 and MTBS-2 (upper). The corresponding protofilaments of the native MTD are labeled in the attached model (lower). (b) The structure of MTD tubulins (16-nm repeat) locally refined to 3.08 Å resolution. (c) The inter-PF angles of the MTD lattice in this study show the same pattern as that of previously published ones in both T. thermophila (PDB: 6U0H)³⁶ and C. reinhardtii (PDB: 6U42)¹⁵. Each inter-PF angle was calculated three times. Data are presented as mean values ± SD. (d-e) The local density map of α-MTBD/LC1/tubulins complex was lowpass filtered to 6 Å to clearly show the density connection between β-CTT with LC1 (d) and γ-flap (e).