Extended Data Fig. 8. The structure of free OAD in pre-parallel state.

(a) The representative negative staining image of the free OADs. Similar images could be regularly acquired from n > 5 independent OAD purifications. Scale bar, 100 nm (b) Representative two-dimensional (2D) projection of the free OAD sample with a model attached for each row. The three motor domains and tail region have a wide range of dynamic conformations. Scale bar, 10 nm. (c) The representative cryo-EM image of the free OADs. Similar images could be regularly acquired from n > 5 independent OAD purifications. Scale bar, 100 nm. (d) Representative 2D averages of the free OAD sample purified via Grafix⁵⁷. Scale bar, 10 nm. (e) The cryo-EM structure of pre-parallel OAD. The atomic model of parallel OAD is fitted into the density map of pre-parallel OAD (transparency grey, upper left) as a rigid body. The cryo-EM density map of pre-parallel OAD is presented at the right panel from two different views. The local density map of the β-HB4-9 is attached. (f) The local resolution, FSC curve, and orientational distribution for the representative region from the free OAD.