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. 2021 Sep 23;28(10):799–810. doi: 10.1038/s41594-021-00656-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — The 3D classification identified two major microtubule-binding states (MTBS-1 and MTBS-2). The six good classes enclosed in the green rectangle are categorized into MTBS-1. The class in the red square was removed from the dataset for all following data processing. Three classes enclosed in sky blue rectangle are categorized into MTBS-2. Multiple levels of masks were implemented in the local refinement to improve the resolution of OAD-PF structure. The box size (~680 nm) was optimized to cover at least two adjacent OADs and the four PFs they bind to. The attached images represent typical local densities of the 3D volumes generated from cryoSPARC 2.12.