Table 1.
Titer, total virus particles and ratio of defective particles: infectious virions of HCoV-OC43 propagated in HRT-18, MRC-5 and Vero E6 cells.
| Cell Line | Day | Log10 TCID50/mL | Std. Error | Virus particles/mL | Std. Error | Total Particles | Infectious Particles | Defective Particles | Defective particles: Infections virions |
|---|---|---|---|---|---|---|---|---|---|
| HRT-18 | 1 | 5.15 | 0.07 | 6.77 | 0.07 | 5.89E+06 | 1.40E+05 | 5.75E+06 | 41.1 |
| HRT-18 | 2 | 5.59 | 0.13 | 7.18 | 0.21 | 1.51E+07 | 3.90E+05 | 1.47E+07 | 37.8 |
| HRT-18 | 3 | 5.86 | 0.28 | 7.87 | 0.04 | 7.41E+07 | 7.21E+05 | 7.34E+07 | 101.7 |
| HRT-18 | 4 | 5.69 | 0.07 | 8.21 | 0.01 | 1.62E+08 | 4.91E+05 | 1.62E+08 | 329.4 |
| HRT-18 | 5 | 6.40 | 0.39 | 8.37 | 0.03 | 2.34E+08 | 2.50E+06 | 2.32E+08 | 92.9 |
| HRT-18 | 6 | 6.47 | 0.10 | 8.37 | 0.05 | 2.34E+08 | 2.98E+06 | 2.31E+08 | 77.6 |
| HRT-18 | 7 | 6.48 | 0.20 | 8.46 | 0.05 | 2.88E+08 | 3.03E+06 | 2.85E+08 | 94.3 |
| Cell Line | Day | Log10 PFU/mL | Std. Error | Virus particles/mL | Std. Error | Total Particles | Infectious Particles | Defective Particles | Defective particles: Infections virions |
|---|---|---|---|---|---|---|---|---|---|
| MRC-5 | 1 | 6.75 | 0.08 | 6.59 | 0.02 | 3.86E+06 | 5.67E+06 | −1.81E+06 | N/A* |
| MRC-5 | 2 | 6.92 | 0.07 | 7.45 | 0.07 | 2.80E+07 | 8.25E+06 | 1.98E+07 | 2.4 |
| MRC-5 | 3 | 6.89 | 0.08 | 7.24 | 0.07 | 1.73E+07 | 7.76E+06 | 9.50E+06 | 1.2 |
| MRC-5 | 4 | 7.02 | 0.07 | 7.18 | 0.08 | 1.51E+07 | 1.04E+07 | 4.69E+06 | 0.5 |
| MRC-5 | 5 | 6.59 | 0.15 | 7.17 | 0.03 | 1.49E+07 | 3.86E+06 | 1.10E+07 | 2.9 |
| MRC-5 | 6 | 6.51 | 0.06 | 7.09 | 0.01 | 1.23E+07 | 3.26E+06 | 9.06E+06 | 2.8 |
| MRC-5 | 7 | 6.07 | 0.03 | 7.43 | 0.12 | 2.71E+07 | 1.17E+06 | 2.59E+07 | 22.1 |
| Cell Line | Day | Log10 TCID50/mL | Std. Error | Virus particles/mL | Std. Error | Total Particles | Infectious Particles | Defective Particles | Defective particles: Infections virions |
|---|---|---|---|---|---|---|---|---|---|
| Vero E6 | 1 | 4.83 | 0.22 | N/A | N/A | N/A | N/A | N/A | N/A |
| Vero E6 | 2 | 5.38 | 0.12 | N/A | N/A | N/A | N/A | N/A | N/A |
| Vero E6 | 3 | 5.67 | 0.07 | N/A | N/A | N/A | N/A | N/A | N/A |
| Vero E6 | 4 | 6.55 | 0.04 | 6.95 | 0.38 | 8.93E+06 | 3.55E+06 | 5.39E+06 | 1.5 |
| Vero E6 | 5 | 6.71 | 0.21 | 7.65 | 0.33 | 4.44E+07 | 5.10E+06 | 3.93E+07 | 7.7 |
| Vero E6 | 6 | 6.47 | 0.04 | 8.14 | 0.18 | 1.39E+08 | 2.97E+06 | 1.36E+08 | 46.0 |
| Vero E6 | 7 | 6.51 | 0.13 | 8.51 | 0.10 | 3.21E+08 | 3.25E+06 | 3.18E+08 | 97.9 |
*
Value not able to be determined because Virus Particles/mL were less than PFU/mL.