Table 2.
Summary of the re-evaluated histopathologic findings for tumor samples with available material
| # | Detailed institutional histopathological diagnosis | Cell density | Cytoplasm and nuclei pleomorphism | Mitotic figures/10 HPF | Microvascular proliferation | Necrosis | Infiltration | Notes/fusion detected |
|---|---|---|---|---|---|---|---|---|
| 4 | Pleomorphic xanthoastrocytoma | High | Slightly pleomorphic, focally spindle cells | 0 | Hyalinized | No | EWSR1:PATZ1, perivascular pseudorosettes, biphasic differentiation | |
| 10 | Glioblastoma WHO grade 4 | High | Monomorphous nuclei, single giant cells | 2 | Few | No | Glial | |
| 12 | Anaplastic ependymoma WHO grade 3 | High | Monomorphous nuclei | 1 | Yes | Yes | Pseudorosettes | |
| 13 | N/A | Moderate | Monomorphous, small, arranged in groups/lobules | 0 | Hyalinized | No | Microcysts, biphasic differentiation | |
| 14 | Malignant NET with focal glial and sarcomatous differentiation | High | Small cell, partially spindle-shaped | 0 | No | No | MN1:PATZ1, biphasic differentiation | |
| 22 | Giant cell glioblastoma WHO grade 4 | High | Pleomorphic, small and large cells | 0 | Yes | No | MN1:PATZ1, biphasic differentiation | |
| 23 | Glioblastoma WHO grade 4 | High | Focally very pleomorphic, giant cells | 3 | Hyalinized | No | Diffuse | Focally spindle cells, biphasic differentiation |
| 24 | Low-grade glial/glioneuronal tumor | High | Pleomorphic, small and large cells | 0 | Yes | No | Diffuse | EWSR1:PATZ1 |
| 25 | Malignant neuroepithelial tumor | High | Monomorphous nuclei | 0 | Yes | No | EWSR1:PATZ1, microcysts | |
| 28 | Pleomorphic xanthoastrocytoma | High | Monomorphous, single giant cells, focally spindle cells | 0 | Few | No | Strong infiltration | MN1:PATZ1 |
| 30 | High-grade glioma | High | Pleomorphic, focally spindle-shaped, many apoptotic bodies | 8 | Yes | Yes | MN1:PATZ1, focally perivascular pseudorosettes, biphasic differentiation | |
| 39 | Anaplastic clear cell ependymoma WHO grade 2 | High | Monomorphous, clear cells | 0 | Yes | No | Ependymal, pseudorosettes | |
| 40 | Anaplastic ependymoma WHO grade 3 | High | Monomorphous nuclei | 0 | Yes | Yes | EWSR1:PATZ1, astroblastoma-like | |
| 46 | High-grade glioma | Moderate/high | Fibrillary, monomorphous nuclei | 0 | Yes | Yes | Diffuse | MN1:PATZ1, pseudorosettes, astroblastoma-like |
| 54 | Glioblastoma WHO grade 4 | High | Monomorphous, small clusters of cells | 1 | Yes | No | Clusters | Infiltration in small clusters of cells |
| 55 | N/A | High | Moderate nuclear pleomorphism | 1 | Yes | Yes | MN1:PATZ1, pseudorosettes | |
| 56 | Glioblastoma WHO grade 4; Gliosarcoma | High | Spindle-shaped cells | 0 | Yes | No | Sarcomatous differentiation | |
| 71 | Glioblastoma WHO grade 4 | High | Slightly pleomorphic, focally clear cells, prominent nucleoli | 5 | Hyalinized | Yes | EWSR1:PATZ1, perivascular pseudorosettes, focally spindle cells, biphasic differentiation |
Details about immunohistochemistry is listed in Supplementary Table 1
N/A not applicable