KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| E. coli Rosetta (DE3)pLysS | EMD Millipore | Cat#70956-3 |
| ScarabXpress T7lac | (Scarab Genomics) | Cat#C-1709-05K |
| Spodoptera frugiperda (Sf9) cells | Invitrogen | Cat#11496015 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| SpNatC (Naa3531−708 /SpNaa30FL /SpNaa3848−116) | (Liszczak et al., 2011) This Paper | N/A |
| Binary ScNatC (ScNaa301−161 /ScNaa3519−733) | (Liszczak et al., 2013) This Paper | N/A |
| Ternary ScNatC (ScNaa301−161 /ScNaa3519−733/ScNaa381−70) | This Paper | N/A |
| SpNatA (SpNaa15/Naa101−156) | (Liszczak et al., 2013) | N/A |
| hNatB (hNAA251−972/hNAA201−163) | (Deng et al., 2020b) | N/A |
| Nickel Resin | Thermo Scientific | Cat#88223 |
| HiTrap SP HP, 5 mL | GE Healthcare | Cat#17115201 |
| Superdex 200 10/300 GL | GE Healthcare | Cat#17517501 |
| [14C]Acetyl-CoA (4 mCi/mmol) | PerkinElmer Life Sciences | Cat#NEC313050UC |
| P81 Phosphocellulose squares | St. Vincent’s Institute Medical Research | N/A |
| MLGP Peptide (NH2-MLGPEGGRWGRPVGRRRRP-COOH) | Genscript | N/A |
| SASE Peptide (NH2-SASEAGVRWGRPVGRRRRP-COOH) | Genscript | N/A |
| MDVF Peptide (NH2-MDVFMKGRWGRPVGRRRRP-COOH) | Genscript | N/A |
| SGRG Peptide (NH2-SGRGKGGKGLGKGGAKRHR-COOH) | Genscript | N/A |
| MLRF Peptide (NH2-MLRFVTKRWGRPVGRRRRP-COOH) | Genscript | N/A |
| Deposited Data | ||
| Atomic coordinates and structure factors (ternary SpNatC) | This paper | PDB: 7L1K |
| Atomic coordinates and structure factors (hNaa50) | (Liszczak et al., 2011) | PDB: 3TFY |
| Atomic coordinates and structure factors (hNatA) | (Gottlieb and Marmorstein, 2018) | PDB: 6C9M |
| Atomic coordinates and structure factors (SpNatA) | (Liszczak et al., 2013) | PDB: 4KVM |
| Atomic coordinates and structure factors (hNatB) | (Deng et al,, 2020) | PDB: 6VP9 |
| Cryo-EM map (ternary SpNatC) | This paper | EMD-23110 |
| Oligonucleotides | ||
| Primers for recombinant NatC mutants | This paper | Supplementary Table 1 |
| Recombinant DNA | ||
| pET DUET vector- His6-SUMO- | This paper | N/A |
| pET DUET vector- His6-TEV- | This paper | N/A |
| Software and Algorithms | ||
| Phenix | (Adams et al., 2010) | https://www.phenix-online.org/documentation/reference/refinement.html |
| RELION | (Zivanov et al., 2018) | N/A |
| MotionCor2 | (Zheng et al., 2017) | N/A |
| CryoSPARC | (Punjani et al., 2017) | N/A |
| Coot | (Emsley and Cowtan, 2004) | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| PyMOL | Schrodinger LLC | http://www.pymol.org |
| Prism 5.0 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Clustal Omega | (Sievers et al., 2011) | https://www.ebi.ac.uk/Tools/msa/clustalo/ |
| ESPript 3.0 | (Robert and Gouet, 2014b) | http://espript.ibcp.fr/ESPript/ESPript/ |
| LigPlot+ | (Laskowski and Swindells, 2011) | https://www.ebi.ac.uk/thornton-srv/software/LigPlus/ |