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. Author manuscript; available in PMC: 2022 Oct 7.
Published in final edited form as: Structure. 2021 May 20;29(10):1094–1104.e4. doi: 10.1016/j.str.2021.05.003

Table 2.

SpNatC complex (WT and mutant SpNaa30) catalytic parameters

Substrate Protein (SpNatC) kcat* (min−1) Relative kcat (normalized to WT) Km* (μM) Relative Km (normalized to WT) kcat/Km (normalized to WT)
Gag
Peptide
(MLRF-)
WT 19.7 ± 3.6 1.0 7.8 ± 1.4 1.0 1.0
S24A 19.1 ± 2.8 0.97 8.9 ± 1.0 1.1 0.85
E25A 33.6 ± 11 1.7 11.1 ± 6.1 1.42 1.2
Y27A 8.7 ± 1.6 0.44 2.5 ± 0.4 0.32 1.4
Y71A 32.7 ± 8.5 1.7 10.7 ± 3.8 1.4 1.2
Y71F 18.4 ± 8.3 0.94 13.9 ± 11 1.8 0.52
M74A 13.3 ± 1.9 0.68 11.4 ± 5.2 1.5 0.46
E109A 10.4 ± 0.6 0.53 15.2 ± 9.6 1.95 0.27
E109Q 2.4 ± 0.4 0.12 72.1 ± 95 9.25 0.013
N114A 10.9 ± 1.9 0.55 4.4 ± 0.9 0.56 0.99
Y121A 1.8 ± 0.7 0.093 9.7 ± 5.0 1.2 0.075
Y121F 1.8 ± 0.5 0.090 5.3 ± 2.0 0.68 0.13
Y135A 4.1 ± 1.3 0.21 4.0 ± 3.9 0.51 0.41
Y136A 0.2 ± 0.1 0.011 0.7 ± 0.4 0.091 0.13
*

values indicated mean ± S.D. of three independent kinetic curves fitting results.

See Supplementary Figure.6, for the raw curves.