Fig. 6.
Effect of GluD1 ablation from dorsal striatum on behavior. A. Experimental timeline, GluD1flox/flox mice received bilateral micro injections of AAV-eGFP and AAV-eGFP-Cre (Day 1) into the dorsal striatum (DS), followed by behavioral assays (Day 28–50). B and B’. Representative track plots of the path travelled by mice (DS-AAV-eGFP and DS-AAV-eGFP-Cre). C. DS-AAV-eGFP-Cre group showed significantly more distance traveled compared to the DS-AAV-eGFP group (DS-AAV-eGFP 46.83 ± 2.954 (N = 7) vs. DS-AAV-eGFP-Cre 59.98 ± 4.406 (N = 7), p = 0.0316, unpaired t-test). C’. Locomotor activity per 5 min revealed a significant effect of GluD1 deletion ([F (1, 12)=6.143, p = 0.0290], Two-way ANOVA, Bonferroni’s post-hoc test, p = 0.0111). D and D’. DS-AAV-eGFP-Cre group showed significantly more time spent in open arm of zero maze (DS-AAV-eGFP 33.45 ± 6.487 (N = 10) vs. DS-AAV-eGFP-Cre 52.25 ± 5.365 (N = 12), p = 0.0382, unpaired t-test) and more number of entries (DS-AAV-eGFP 7.5 ± 1.222 vs DS-AAV-eGFP-Cre 13.25 ± 2.06, p = 0.0278, unpaired t-test). E. No change in percent alternation in the Y-maze test (N = 7, p > 0.9999, unpaired t-test). F. In marble burying test, DS-AAV-eGFP-Cre group buried significantly fewer marbles (DS-AAV-eGFP 10.55 ± 1.098 (N = 11) vs. DS-AAV-eGFP-Cre 3.909 ± 1.516 (N = 11), p = 0.0020, unpaired t-test). G. In the sociability test no significant difference in time interacting with the stranger mouse (animate) (N = 13–14, p = 0.9813, unpaired t-test) and inanimate chamber (p = 0.5120, unpaired t-test). H. In novel object recognition test no change in short-term (N = 6– 7, p = 0.5672, unpaired t-test) or long-term memory (p = 0.1982, unpaired t-test). I. In a forced swim test no significant difference in mobility events (N = 9–10, p = 0.3651, unpaired t-test) and immobility events (p > 0.9999, unpaired t-test). All data are presented as mean ± SEM