Fig. 7.
Effect of GluD1 ablation from PV neurons on anxiety- and depression-like behavior. A. Breeding strategy and experimental timeline, GluD1flox/flox mice were crossed with the PV-Cre mice to produce PV-GluD1 KO. Behavioral experiments were conducted on Day 65–90. B and B’. Representative track plots of the path travelled by mice (PV-Cre and PV-GluD1 KO). C. PV-GluD1 KO mice did not show any significant change in distance traveled compared to the PV-Cre mice (N = 8–9, p = 0.5633, unpaired t-test). C’. Further the locomotor activity per 5 min was also similar between groups. D and D’. No change in time spent in open arm (N = 5, p = 0.2387, unpaired t-test) or number of entries (p = 0.3597, unpaired t-test). E. No change in percent alternation in Y-maze test (N = 8–9, p = 0.8400, unpaired t-test). F. In marble burying test, PV-GluD1 KO mice buried significantly fewer marbles compared to the PV-Cre mice (PV-Cre 9.6 ± 1.122 (N = 5) vs. PV-GluD1 KO 3.8 ± 1.463 (N = 5), p = 0.0137, unpaired t-test). G. In the sociability test, PV-GluD1 KO mice showed significantly less interaction time with the stranger mouse (animate) (PV Cre 126.9 ± 5.582 (N = 5) vs. PV-GluD1 KO 53.58 ± 11.11 (N = 5), p = 0.0004, unpaired t-test) compared to the PV-Cre mice. No difference in inanimate chamber exploration between groups (p = 0.2072, unpaired t-test). H. In novel object recognition test no change in short-term (N = 8–9, p = 0.3363, unpaired t-test) or long-term memory (p = 0.8506, unpaired t-test). I. In a forced swim test PV-GluD1 KO mice showed significantly more mobility events (PV-Cre 28.6 ± 2.272 (N = 5) vs. PV-GluD1 KO 38.8 ± 2.289 (N = 5), p = 0.0133, unpaired t-test) and less immobility events (PV-Cre 43.2 ± 2.396 vs. PV-GluD1 KO 33.4 ± 2.337 p = 0.0190, unpaired t-test) compared to PV-Cre mice. All data are presented as mean ± SEM