KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit Polyclonal Biotinylated Anti-GFP | Thermo Fisher Scientific | Cat#A10259; RRID: AB_2534021 |
| Rabbit Polyclonal Anti-PARP1 | Abcam | Cat#Ab227244 |
| Mouse Monoclonal Anti-PARP1 | Atlas Antibodies | Cat#AMAb90959; RRID: AB_2665732 |
| Rabbit Monoclonal Anti-SUMO1 | Abcam | Cat#Ab32058; RRID: AB_778173 |
| Rabbit Polyclonal Anti-XPO1 | Atlas Antibodies | Cat#HPA042933; RRID: AB_2678229 |
| Rabbit Polyclonal Anti-MATR3 | Atlas Antibodies | Cat#HPA036565; RRID: AB_10673623 |
| Mouse Monoclonal Anti-IPO5 | Santa Cruz Biotechnologies | Cat#sc-55527; RRID: AB_2127684 |
| Rabbit Polyclonal Anti-LEO1 | Thermo Fisher Scientific | Cat#A300-175A; RRID: AB_2135932 |
| Mouse Monoclonal Anti-PARP1 | Thermo Fisher Scientific | Cat#MA3-950; RRID: AB_325523 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Transcend™ tRNA | Promega | Cat#L5061 |
| BS3 | Thermo Fisher Scientific | Cat#A39266 |
| Critical Commercial Assays | ||
| PURExpress® In Vitro Protein Synthesis Kit | NEB | Cat#E6800S |
| SuperScript™ III One-Step RT-PCR System | Invitrogen | Cat#12574018 |
| NEBNext® Ultra™ II FS DNA Module | NEB | Cat#E7810S |
| NxSeq® UltraLow DNA Library Kit | Lucigen | Cat#15012-1 |
| Duolink™ In Situ Red Starter Kit Mouse/Rabbit | Millipore Sigma | Cat#DUO92101-1KT |
| Deposited Data | ||
| All sequencing data have been uploaded to GEO. | This paper | GSE150818 |
| Experimental Models: Cell Lines | ||
| Human T-Cells (Jurkat) | ATCC | Cat#TIB-152; RRID: CVCL 0255 |
| Human Embryonic Kidney Cells (HEK 293T) | ATCC | Cat#CRL-3216; RRID: CVCL_0063 |
| Human Umbilical Vein Cells (HUVEC) | ATCC | Cat#CRL-1730; RRID: CVCL_2959 |
| Experimental Models: Organisms/Strains | ||
| Oligonucleotides | ||
| Right/Random primer: 5′ TTT CCC CGC CGC CCC CCG TCC TGC TGC CGC CCT TGT CGT CAT CGT CTT TGT AGT C(Nx15) 3′ | This paper | N/A |
| Library TSO: 5′ /5Biosg/GGC TCA CGA GTA AGG AGG ATC CAA CAT rGrGrG 3′ | This paper | N/A |
| Left PCR primer: 5′ GCG AAT TAA TAC GAC TCA CTA TAG GGC TCA CGA GTA AGG AGG 3′ | This paper | N/A |
| Right PCR primer: 5′ TTT CCC CGC CGC CCC CCG TC 3′ | This paper | N/A |
| Biotin Arm (w/dI): 5’ /5Phos/CC/ideoxyI/ C/iBiodT/C /ideoxyI/AC CCC CCG CCC CCC CCG /iAzideN/CCT 3’ | This paper | N/A |
| Biotin Arm (w/o dI): 5’ /5Phos/CCG C/iBiodT/C GAC CCC CCG CCC CCC CCG /iAzideN/CCT 3’ | This paper | N/A |
| Puromycin Arm: 5’ /5DBCON/TCT /iSp18/iSp18/iSp18/iSp18/CC/3Puro/ 3’ | This paper | N/A |
| Puromycin containing linker with no biotin: 5’ /5Phos/CC/ideoxyI/CTC/ideoxyI/ACCCCCCGCCGCCCCCCGTCCT/iSp18/iSp18/iSp18/iSp18/CC/3Puro/ 3’ | This paper | N/A |
| Universal forward primer: 5’ AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCGAGTAAGGAGGATCCAACATG 3’ | This paper | N/A |
| Indexed reverse primer: 5’ CAAGCAGAAGACGGCATACGAGATXXXXXXXXGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCTTGTCGTCATCGTCTTTGTAGTC 3’, where X represents the index bases | This paper | N/A |
| End Capture TSO: 5’ /5dSp/AGT AAA GGA GAC CTC AGC TTC ACT GGA rGrGrG 3’ | This paper | N/A |
| Recombinant DNA | ||
| Software and Algorithms | ||
| PROPERseqTools is available at https://github.com/Zhong-Lab-UCSD/PROPERseqTools. | This paper | DOI: 10.5281/zenodo.5009171 |
| other | ||