FIGURE 4.

P22077 significantly inhibits melanoma tumour growth in vivo. (A) A375 melanoma cells were xenografted into nude mice. The mice were randomized for intraperitoneal injection of P22077 (10 mg/kg) or control as indicated time‐points for 12 days. Right panel: Photograph of the tumours from the mice after the mice were euthanized at the treatment end‐point. The bodyweight ((B) and tumour growth (C) were measured during the treatment period. (D) The tumour volumes in the last day. (E) Tumours were removed at the treatment end‐point, and each tumour mass from the mice was weighed (mean values ± SEM, n = 4). Significant differences were evaluated using a one‐way ANOVA. *p < 0.05 vs control group. Tissue sections from the tumour were fixed and stained with haematoxylin and eosin (F) and TUNEL (G) to examine the tumour histochemical morphology of apoptosis. The nucleus was stained with DAPI. Scale bar is 10 µm