FIGURE 2.

LINC00511 facilitates GC cell migration, EMT, stemness and GC tumour growth. A, Transwell assay detected number of migrated GC cells by knockdown of LINC00511. B, Western blot assay was conducted to detect the expression of relevant proteins in LINC00511‐silenced cells. C, Immunofluorescence staining detected E‐cadherin and N‐cadherin positivity by LINC00511 depletion. D, Sphere formation assay was implemented to detect sphere formation efficiency in GC cells. E, qRT‐PCR and Western blot assays detected the mRNA and protein levels of stemness biomarkers including OCT4, Nanog and SOX2 in GC cells. F, Volume and weight of xenograft tumours by LINC00511 depletion were demonstrated. G, We adopted qRT‐PCR and Western blot assays to detect the protein and mRNA levels of stemness biomarkers in vivo. H, Immunohistochemical staining assay revealed Ki‐67, PCNA, SOX2, E‐cadherin and N‐cadherin positivity by LINC00511 knockdown in vivo. **P < .01