FIGURE 3.

LINC00511 recruited EZH2 to PTEN promoter and facilitated methylation of PTEN promoter. A, Subcellular fraction assay and FISH assay detected subcellular location of LINC00511 in GC cells. B, We adopted qRT‐PCR and Western blot assays to detect mRNA and protein levels of PTEN by LINC00511 knockdown. C, The luciferase activity of PTEN promoter in LINC00511‐silenced cells was detected. D, ChIP assay was conducted to detect the enrichment of PTEN promoter in GC cells after transfection with sh‐NC or sh‐LINC00511#1. E, Silver staining and Western blot disclosed that EZH2 protein was pulled down by LINC00511. F, RIP assay revealed the enrichment of PTEN. G, The depletion efficiency of sh‐EZH2 was detected by RT‐qPCR. H, Influence of LINC00511 depletion on EZH2 and that of EZH2 depletion on LINC00511 was evaluated. I, qRT‐PCR examined the influence of EZH2 depletion on PTEN. J, ChIP assay of PTEN promoter enriched in anti‐IgG and anti‐H3K27me3 in GC cells after transfection with sh‐NC or sh‐EZH2#1. **P < .01. The symbol ‘n.s’ represents no significance