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. 2021 Aug 24;25(19):9112–9127. doi: 10.1111/jcmm.16656

FIGURE 5.

FIGURE 5

LINC00511 served as a ceRNA to up‐regulate SOX4 via sponging miR‐195‐5p. A, We adopted qRT‐PCR assay to disclose the relative expression of different miRNAs in GC cells compared to normal cells. B, RNA pull‐down assay tested relative enrichment of miR‐29c‐3p and miR‐195‐5p pulled down by biotinylated LINC00511. C, ENCORI database predicted the binding sites between LINC00511 and miR‐195‐5p. D, We detected miR‐195‐5p expression in GC cells transfected with miR‐195‐5p‐mimics through qRT‐PCR assay. E, Luciferase activity of wild‐type and mutant LINC00511 vectors was detected. F, We utilized qRT‐PCR assay to detect SOX4 expression in GC cells and in normal cells. G, Binding sites between SOX4 and miR‐195‐5p were revealed based on ENCORI database. H, The luciferase activity of wild‐type and mutant LINC00511 vectors was detected. I, RIP assay of miR‐195‐5p, LINC00511 and SOX4 in cells treated with antibodies against IgG and Ago2. J, We adopted qRT‐PCR assay to detect the relative expression of SOX4 in GC cells in different groups. *P < .05 and **P < .01. The symbol ‘n.s’ represents no significance