FIGURE 1.

Beclin1 expression was upregulated in the alveolar tissue of mice with CS‐induced silicosis. (A) HE and Sirius red staining of alveolar tissues of mice with CS‐induced silicosis and control mice. (B) RT‐qPCR analysis of Col1a1 and Col3a1 mRNA levels in alveolar tissues of mice with CS‐induced silicosis and control mice. (C) RT‐qPCR and Western blot analysis of Beclin1 mRNA and protein levels in alveolar tissues on day 3 and day 56 after CS induction or saline treatment at the same time points. (D) IHC analysis of Beclin1 in mouse alveolar tissues day 56 after CS treatment. (E) IHC analysis of Beclin1 in clinical diseased tissues of silicosis and normal tissues. Panels A‐E, *p < 0.05, **p < 0.01 and ***p < 0.001, compared with mice treated with saline. Measurement data were analysed by unpaired t test between two groups