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. 2021 Aug 24;25(19):9214–9227. doi: 10.1111/jcmm.16825

FIGURE 2.

FIGURE 2

Beclin1 promotes autophagy of CS‐induced AMs. (A) Detection of Beclin1, LC3 and ATG5 in CS‐induced MH‐S cells treated with rapamycin or 3MA. (B) Detection of Beclin1 expression pattern after transfection with oeBeclin1 vector, siBeclin1 or control in MH‐S cells. (C) Detection of LC3 and ATG5 after transfection with oeBeclin1 vector, siBeclin1 or control in MH‐S as well as quantification of LC3‐II/LC3‐I ratio. (D) The distribution of GFP and RFP observed under a confocal microscope after treating MH‐S cells with mRFP‐GFP‐LC3 recombinant adenovirus and CS. (E) The number of autolysosomes/autophagosomes counted under a confocal microscope after the cells in D were with oeBeclin1 vector, siBeclin1 or control followed by CS treatment. *p < 0.05; **p < 0.01; ***p < 0.001 and ****p < 0.0001. Measurement data were analysed by unpaired t test between two groups. The experiment was conducted 3 times independently