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. 2021 Aug 24;25(19):9214–9227. doi: 10.1111/jcmm.16825

FIGURE 5.

FIGURE 5

E2F1 is the potential target of miR‐205‐5p. (A) Screening of miRNAs potentially regulating E2F1 through starBase, TargetScan and miRDB database. (B) Quantification of miR‐17‐5p/miR‐20‐5p/miR‐93‐5p/miR‐106‐5p/miR‐205‐5p/miR‐519‐3p in alveolus tissues of mice with silicosis. **p < 0.01 vs. mice treated with saline; n.s., no significance. (C) Quantification of miR‐205‐5p and E2F1 after transfection of miR‐205‐5p mimic or inhibitor in 293T and MH‐S cells. **p < 0.01 and ***p < 0.001 vs. 293T/MH‐S cells treated with Ctrl mimic/inhibitor. (D) Detection of the luciferase activity after co‐transfection of luciferase reporter gene vector and miR‐205‐5p mimic or inhibitor in 293T and MH‐S cells. **p < 0.01 and ***p < 0.001 vs. 293T/MH‐S cells treated with Ctrl mimics/inhibitor; n.s., no significance. Measurement data were analysed by unpaired t test between two groups. The experiment was conducted 3 times independently