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. 2021 Aug 24;25(19):9214–9227. doi: 10.1111/jcmm.16825

FIGURE 6.

FIGURE 6

The E2F1/SKP2/Beclin1 axis is a necessary link for miR‐205‐5p to promote autophagy of AMs. A‐B, The expression patterns of miR‐205‐5p, E2F1 and SKP2 mRNAs (A) as well as E2F1 and SKP2 proteins (B) in CS‐induced MH‐S cells treated with rapamycin or 3MA. C, Detection of SKP2 and Beclin1 in MH‐S cells after transfection with miR‐205‐5p mimic or inhibitor. D‐E, Detection of E2F1, SKP2 and Beclin1 (D) as well as LC3‐II/LC3‐I ratio and ATG5 (E) in MH‐S cells transfected with oeE2F1/oeSKP2 or siBeclin1 and miR‐205‐5p mimic. (F) Quantification of the numbers of LC3 puncta and mRFP fluorescent spots after CS‐induced MH‐S cells were treated with recombinant adenovirus mRFP‐GFP‐LC3, miR‐205‐5p mimic, oeE2F1/oeSKP2 vectors, or siBeclin1. *p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001. Measurement data were analysed by unpaired t test between two groups and one‐way ANOVA among multiple groups. The experiment was repeated 3 times independently