FIGURE 2.

CXC chemokine ligand‐13 promotes cell mobility in lung cancer cells. (A‐C) The CL1‐0 lung cancer cells were incubated with various concentrations (0, 3, 10 and 30 ng/mL) of human recombinant CXCL13 for 24 h, and the migration A, or invasion assay B, were performed by using Transwell. The migrating and invading cells were counted by microscope. C, The cell viability of CL1‐0 cells in response to CXCL13 incubation was assessed by CCK8 reagent. D and E, The CL1‐0 cells were transfected with CXCL13 shRNA for 24 h, and the knockdown efficiency of CXCL13 was confirmed by Western blot and qPCR. F, The CL1‐0 transfected with CXCL13 was subjected to cell migration assay. Results are expressed as the mean ± SD of triplicate samples. *P < .05 compared to the control group