FIGURE 6.

CCAT1 enhanced proliferation and angiogenesis in vitro and faciliated tumorigenesis and metastasis in vivo.(A) EdU assay were used to evaluate cell proliferation under CCAT1 overexpression or the co‐transfection of pcDNA3.1‐CCAT1 and siFABP5. (B) Tube formation assay was carried out to assess the angiogenesis ability of HUVECs under CCAT1 overexpression or the co‐transfection of pcDNA3.1‐CCAT1 and siFABP5. Results analysed by one‐way ANOVA. (C‐D) Xenograft assay was carried out with lv‐CCAT1 transfected mice with lv‐vector as control. Tumour volume and Tumour weight were measured. Results analysed by two‐way ANOVA. (E‐H) The effect of CCAT1 on number of metastatic nodes and lung weight were evaluated. Three biological replicates each group for all experiments