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. 2021 Oct 8;12:5904. doi: 10.1038/s41467-021-26125-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — IPO13^+/+ ESCs were treated without or with siRNAs targeting SP1 or KLF4. 3 d post transfection, cells were treated with H₂O₂ as indicated for 1 h, and then stained either immediately (“No recovery”) or subsequent to 2 h recovery as indicated, for propidium iodide (PI) and flow cytometric analysis. a Representative dot plots of the indicated samples with the percentage of ESC PI positive. b Pooled data (IPO13^+/+ 125 μM (n = 7), IPO13^+/+ 300–600 μM (n = 9), IPO13^−/− 125–600 μM (n = 9), IPO13^+/+ +KLF4 siRNA 125–600 μM (n = 7), IPO13^+/+ + SP1 siRNA 125–600 μM (n = 7)) for % PI positive cells relative to untreated (UT) control from the analysis such as that in a are shown (mean ± SEM), where p values (two tailed student’s t test) top to bottom left to right are no recovery: p = 0.6160, p = 0.0032, p = 0.0210, p = 0.0215, p = 0.0198, p = 0.0029, p = 0.0048, p = 0.0326, p = 0.0074, and 2 h recovery: p = 0.6160, p = 0.3882, p = 0.0273, p = 0.6300, p = 0.6484, p = 0.0123, p = 0.1919, p = 0.3598, p = 0.0278. Source data are provided as a Source Data file.