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. 2021 Oct 8;12:5911. doi: 10.1038/s41467-021-26134-w

Fig. 1. Adenosine generated in the course of T cell activation prevents an exacerbated T cell response.

Fig. 1

a Schematic representation for the degradation of ATP to adenosine by the ectonucleotidases CD39 and CD73. b–d PBMCs or CD4 T cells were stimulated with αCD3/αCD28 and treated with b the ADA inhibitor EHNA or different concentrations of adenosine receptor agonists c NECA, and d CADO. CD25 expression and proliferation were measured after 3 to 4 days by flow cytometry. Data were shown for b five donors or c, d one representative donor (mean of technical replicates). e, f CD73 and CD39 expression on human T cell subsets in e representative dot plots and f as a summary for ten donors (median). g CD73− CD4con T cells were stimulated with αCD3/αCD28 in the presence of the ADA inhibitor EHNA (10 µM) and incubated with Tregs, ATP (50 µM) and recombinant CD73 (15 ng/mL) as indicated. The ratios of CD4con T cells to Tregs were 1:0.125 to 1:0.5. CD25 expression and proliferation were measured after 4 days by flow cytometry. IFNγ production was determined by ELISA in the cell culture supernatant on day 4. Data were shown for one representative donor out of four analyzed (mean of technical duplicates). A two-tailed paired t-test was used to compare untreated and treated samples in b.