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. 2021 Oct 8;12:5911. doi: 10.1038/s41467-021-26134-w

Fig. 2. Treg-derived CD73 is not essential for adenosine-mediated suppression of conventional CD4 T cells.

Fig. 2

a–d CD4 responder T cells were stimulated with αCD3/αCD28 in the presence of the ADA inhibitor EHNA (10 µM). CD25 expression and proliferation were measured after 4 days by flow cytometry. a CD4con T cells were stimulated and incubated with AMP (50 µM) and Tregs in the indicated ratio. b CD4con T cells were sorted into CD73− and CD73+, the cells were incubated with AMP (50 µM) and the specific CD73 inhibitor PSB-14685 (10 µM). c CD4con CD73− T cells were incubated with AMP (50 µM), the specific CD73 inhibitor PSB-14685 (10 µM), and recombinant CD73 (15 ng/mL). d CD4con CD73− T cells were incubated with AMP (50 µM) and different concentrations of soluble recombinant CD73 (three tenfold serial dilutions starting with 15 ng/mL). PSB-14685 was used to block the highest concentration of recombinant CD73. Data were shown for a three, b, c nine and d five donors (mean ± SD). Ordinary one-way ANOVA with Dunnett’s multiple comparisons test was used to compare all conditions to cells treated with EHNA or EHNA and AMP (first bar).