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. 2021 Oct 8;12:5906. doi: 10.1038/s41467-021-26214-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Validation of antibodies in IMC using a tissue with known staining patterns, an example shown here is a follicle in the spleen; Ir191/193 (blue), B220 (green); CD4 (magenta), CD68 (yellow), αSMA (cyan), vimentin (red). Representative image of two independently stained spleen tissues. b Panel of 27 antibodies that identify multiple cell types from lymphoid, myeloid, tumour and stromal compartments, as well as markers of activation and proliferation status. Detailed information on the antibody clones and isotope conjugations can be found in Supplementary Tables 1 and 2. DC dendritic cells, NK natural killer, EMT epithelial-to-mesenchymal transition, Ag antigen.