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. 2021 Oct 8;11:20053. doi: 10.1038/s41598-021-99585-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibitory effect of SHED administration on the accumulation of activated microglia and reactive astrocytes in the spinal dorsal horn. (a,b) The immunofluorescent CLSM images for IBA1 (a) and GFAP (b) in the spinal dorsal horn 15 days after control medium administration in nerve-injured mice. Scale bar = 100 μm. (c) The columns represent the mean area occupied by IBA1 fluorescence in the SDH. Data represent the mean ± SEM. n = 5. Two-way ANOVA Tukey’s test (**p < 0.01). (d,e) The immunofluorescent CLSM images for IBA1 (c) and GFAP (d) in the dorsal spinal cord 15 days after SHED administration in nerve-injured mice. Scale bar = 100 μm. (f) The columns represent the mean area occupied by IBA1 fluorescence in the SDH. Data represent the mean ± SEM. n = 5. Two-way ANOVA post hoc Tukey’s test (**p < 0.01).