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. 2021 Oct 8;11:20009. doi: 10.1038/s41598-021-99228-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(Top) Mean spectrum containing the NADH spectral contribution with the expected NADH curves fitted (left) and the resultant NADH basis spectrum (right). (Middle) Lipofuscin and flavin spectra fitted to the mean measured spectrum (left) and the resulting basis spectra and their sum (right) which closely matches the mean measured spectrum. (Bottom) The resulting 5 basis spectra from this analysis.

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