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. 2021 Apr 9;26(10):5715–5732. doi: 10.1038/s41380-021-01076-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Characterization of hiPSC-derived neural cultures by immunofluorescence for neuronal marker TUJ1, mature neuron markers MAP2 and NEUN, and astrocyte marker GFAP. B Quantification of secreted soluble Aβ levels in cell supernatant normalized to cellular protein amounts, relative to corresponding E3/3 cultures. n = 15~16 from 3 independent differentiations; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.