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. 2021 Apr 9;26(10):5715–5732. doi: 10.1038/s41380-021-01076-3

Fig. 3. Isolation of pure populations of neurons and astrocytes from mixed cultures.

Fig. 3

A Schematic of magnetic-activated cell sorting (MACS) used to isolate pure neuronal and astrocytic cell populations. Schematic was generated using BioRender. B Representative flow cytometry analysis of CD44 expression in separated CD44− neurons and CD44 + astrocytes. C Immunofluorescence showing expression of mature neuron markers MAP2 and NEUN in neuron, but not astrocyte populations. D Quantification of APOE secretion by astrocyte and neuron populations. E Analysis of calcium transients shows slow calcium transients in the separated astrocytes compared to neuron populations. n = 3~5; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.